Use of 2 brushes: Both brushes supplied in the kit should be used to test the same lesion. The first brush is rotated against the lesion until pinpoint bleeding or pinkish red tissue is observed.
Cells are then transferred to the slide and fixed. The brush is inserted into the vial, brush side down, so that it is submerged in the liquid.
The second brush is introduced to the patient’s mouth to obtain additional cells for the cell block. After brushing, place the second brush directly into the vial (without preparation of a slide) and seal the vial tightly.